Single-gene dual-color reporter cell line to analyze RNA synthesis in vivo.

ABSTRACT: RNA synthesis occurs through the multi-step process of transcription which consists of initiation, elongation, termination, and cleavage of the nascent RNA. In recent years, post-initiation events have attracted considerable attention as regulatory steps in gene expression. In particular, changes in elongation rate have been proposed to alter RNA fate either through changes in RNA secondary structure or recruitment of trans-acting factors, but systematic approaches for perturbing and measuring elongation rate are currently lacking. Here, we describe a system for precisely measuring elongation dynamics for single nascent transcripts at a single gene locus in human cell lines. The system is based on observing the production of fluorescently labeled RNA stem loops which flank a region of inte

SUBMITTER: Palangat M 

PROVIDER: S-EPMC7781226 | biostudies-literature | 2016 Jul

REPOSITORIES: biostudies-literature