Dataset Information

Mitochondrial genome characterization and phylogenetic analysis of bird schistosome Trichobilharzia szidati.

ABSTRACT: In the present study, the complete mitochondrial genome of T. szidati was assembled by next generation sequencing (NGS). We found that the complete mitochondrial genome of T.szidati is 14, 303 bp in length and consists of 3023 (21.1%) adenine, 1153 (8.1%) cytosine, 3432 (24.0%) guanosine and 6695 (46.8%) thymine. The genome contains 12 conserved core protein-coding genes (atp6, cox1, cox2, cox3, nad1, nad2, nad3, nad4, nad4L, nad5, nad6, CYTB), 21 tRNA genes, 2 rRNA genes and 1 D-loop region. Phylogenetic analysis showed that T. szidati has a close relationship with T. regent. Knowledge of mitochondrial genome of T. szidati could provide useful information for the further studies of evolutionary biology, epidemiology and species identification.

SUBMITTER: Wang X

PROVIDER: S-EPMC7781922 | biostudies-literature | 2020 Jun

REPOSITORIES: biostudies-literature

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Mitochondrial genome characterization and phylogenetic analysis of bird schistosome Trichobilharzia szidati.

Wang Xu X Liu Na N

Mitochondrial DNA. Part B, Resources 20200629 3

In the present study, the complete mitochondrial genome of T. szidati was assembled by next generation sequencing (NGS). We found that the complete mitochondrial genome of T.szidati is 14, 303 bp in length and consists of 3023 (21.1%) adenine, 1153 (8.1%) cytosine, 3432 (24.0%) guanosine and 6695 (46.8%) thymine. The genome contains 12 conserved core protein-coding genes (atp6, cox1, cox2, cox3, nad1, nad2, nad3, nad4, nad4L, nad5, nad6, CYTB), 21 tRNA genes, 2 rRNA genes an ...[more]

PMID: 33457871

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Project description:BackgroundCercariae of schistosomes employ bioactive molecules for penetration into their hosts. These are released from specialized unicellular glands upon stimuli from host skin. The glands were previously well-described in the human pathogen Schistosoma mansoni. As bird schistosomes can also penetrate human skin and cause cercarial dermatitis, our aim was to characterize the architecture and ultrastructure of glands in the neurotropic bird schistosome Trichobilharzia regenti and compare it with S. mansoni. In the context of different histolytic enzymes used by these two species, we focused also on the estimations of gland volumes and pH in T. regenti.ResultsThe architecture and 3-D models of two types of acetabular penetration glands, their ducts and of the head gland are shown here. We characterized secretory vesicles in all three gland types by means of TEM and confirmed accuracy of the models obtained by confocal microscopy. The results of two independent approaches showed that the glands occupy ca. one third of cercarial body volume (postacetabular glands ca. 15%, circumacetabular 12% and head gland 6%). The inner environment within the two types of acetabular glands differed significantly as evidenced by dissimilar ability to bind fluorescent markers and by pH value which was higher in circumacetabular (7.44) than in postacetabular (7.08) glands.ConclusionsAs far as we know, this is the first presentation of a 3-D model of cercarial glands and the first exact estimation of the volumes of the three gland types in schistosomes. Our comparisons between T. regenti and S. mansoni implied that the architecture and ultrastructure of the glands is most likely conserved within the family. Only minor variations were found between the two species. It seems that the differences in molecular composition have no effect on general appearance of the secretory cells in TEM. Fluorescent markers employed in this study, distinguishing between secretory vesicles and gland types, can be useful in further studies of mechanisms used by cercariae for host invasion. Results of the first attempts to estimate pH within schistosome glands may help further understanding of regulation of enzymatic activities present within the glands.

Dataset Information

Mitochondrial genome characterization and phylogenetic analysis of bird schistosome Trichobilharzia szidati.

Publications

Mitochondrial genome characterization and phylogenetic analysis of bird schistosome <i>Trichobilharzia szidati</i>.

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