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The complete chloroplast genome sequence of corn and economic analysis on production costs profits in Zhengzhou city.

ABSTRACT: Corn is one of the main food crops in China. The problem of corn production cost benefit has always been the most concerned issue of farmers. In this paper, economic analysis of corn production cost benefit is conducted, and the chloroplast genome sequence is studied. The complete chloroplast genome sequence of corn was characterized from Illumina pair-end sequencing. The chloroplast genome of corn was 140,382 bp in length, containing a large single-copy region (LSC) of 82,156 bp, a small single-copy region (SSC) of 15,952 bp, and two inverted repeat (IR) regions of 21,137 bp. The overall GC content is 36.80%, while the correponding values of the LSC, SSC, and IR regions are 34.5%, 30.5%, and 42.5%, respectively. The genome contains 118 complete genes, including 85 protein-coding genes (78 protein-coding gene species), 25 tRNA genes (19 tRNA species) and 8 rRNA genes (4 rRNA species). The Neighbour-joining phylogenetic analysis showed that corn and Zea mays cultivar B73 clustered together as sisters to other Zea species.

SUBMITTER: Chen Y

PROVIDER: S-EPMC7782024 | biostudies-literature | 2020 Jun

REPOSITORIES: biostudies-literature

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The complete chloroplast genome sequence of corn and economic analysis on production costs profits in Zhengzhou city.

Chen Yiwei Y Ma Yongshuang Y Xu Xueli X

Mitochondrial DNA. Part B, Resources 20200616 3

Corn is one of the main food crops in China. The problem of corn production cost benefit has always been the most concerned issue of farmers. In this paper, economic analysis of corn production cost benefit is conducted, and the chloroplast genome sequence is studied. The complete chloroplast genome sequence of corn was characterized from Illumina pair-end sequencing. The chloroplast genome of corn was 140,382 bp in length, containing a large single-copy region (LSC) of 82,156 bp, a small single ...[more]

PMID: 33457821

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Project description:Understanding the spatial distribution of urban forest ecosystem services is essential for urban planners and managers to effectively manage cities and is an essential part of sustainable urban development. Mapping the spatial distribution of urban forest ecosystem services and improving the accuracy of its assessment scale will undoubtedly provide a more accurate reference basis for later management. In this study, we used the i-Tree Eco model and kriging interpolation to quantify and map urban forest ecosystem services and their spatial distribution in Zhengzhou, a city along the lower reaches of the Yellow River in China; analyzed the mapping errors and applicable conditions; and further explored the spatial differences using geographic probes. The i-Tree Eco model estimation results showed that the total carbon storage in the urban forest of Zhengzhou city was 75.7 tons, the annual carbon sequestration was 14.66 tons, the trees and shrubs in the urban area of Zhengzhou city could effectively avoid a total of 307.86 m3 of surface runoff per year, and trees and shrubs removed 411.8 kg/year of air pollution (O3, CO, NO2, PM2.5, PM10, and SO2). The spatial distribution of all urban forest ecosystem services showed significant heterogeneity, but the spatial evaluation precision of different factors varied. GDP and population data showed a negative correlation with ecosystem services, and ecosystem services were abundant in watershed and woodland areas. This study differs from traditional assessments based on regional data due to its improved spatial evaluation accuracy, and the results, discussion, and analysis not only help Zhengzhou's own urban development, but also provide a basis for the future construction and management of other cities, the Central Plains urban agglomeration, and the surrounding larger regions. This will contribute to the enhancement of ecosystem services and thus improve the ecological conditions of the region. This will also have a positive effect on the health of urban residents.

Project description:Erigeron breviscapus is a famous medicinal plant. However, the limited chloroplast genome information of E. breviscapus, especially for the chloroplast DNA sequence resources, has hindered the study of E. breviscapus chloroplast genome transformation. Here, the complete chloroplast (cp) genome of E. breviscapus was reported. This genome was 152,164bp in length, included 37.2% GC content and was structurally arranged into two 24,699bp inverted repeats (IRs) and two single-copy areas. The sizes of the large single-copy region and the small single-copy region were 84,657 and 18,109bp, respectively. The E. breviscapus cp genome consisted of 127 coding genes, including 83 protein coding genes, 36 transfer RNA (tRNA) genes, and eight ribosomal RNA (rRNA) genes. For those genes, 95 genes were single copy genes and 16 genes were duplicated in two inverted regions with seven tRNAs, four rRNAs, and five protein coding genes. Then, genomic DNA of E. breviscapus was used as a template, and the endogenous 5' and 3' flanking sequences of the trnI gene and trnA gene were selected as homologous recombinant fragments in vector construction and cloned through PCR. The endogenous 5' flanking sequences of the psbA gene and rrn16S gene, the endogenous 3' flanking sequences of the psbA gene, rbcL gene, and rps16 gene and one sequence element from the psbN-psbH chloroplast operon were cloned, and certain chloroplast regulatory elements were identified. Two homologous recombination fragments and all of these elements were constructed into the cloning vector pBluescript SK (+) to yield a series of chloroplast expression vectors, which harbored the reporter gene EGFP and the selectable marker aadA gene. After identification, the chloroplast expression vectors were transformed into Escherichia coli and the function of predicted regulatory elements was confirmed by a spectinomycin resistance test and fluorescence intensity measurement. The results indicated that aadA gene and EGFP gene were efficiently expressed under the regulation of predicted regulatory elements and the chloroplast expression vector had been successfully constructed, thereby providing a solid foundation for establishing subsequent E. breviscapus chloroplast transformation system and genetic improvement of E. breviscapus.

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The complete chloroplast genome sequence of corn and economic analysis on production costs profits in Zhengzhou city.

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The complete chloroplast genome sequence of corn and economic analysis on production costs profits in Zhengzhou city.

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