In Vitro Maturation of Fully Grown Mouse Antral Follicles in the Presence of 1 nM 2-Hydroxyestradiol Improves Oocytes' Developmental Competence.

ABSTRACT: Cathecolestrogens are estradiol metabolites produced during folliculogenesis in the mammalian ovary. 2-Hydroxyestradiol (2-OHE₂) is one of the most abundant although its role remains unknown. The aim of this study is to investigate whether the presence of 2-OHE₂ during the germinal vesicle-to-metaphase II transition affects oocyte meiotic and preimplantation developmental competence. Mouse cumulus-oocyte complexes (COCs), isolated from fully grown antral follicles, were in vitro-matured (IVM) in the presence of 2-OHE₂ (0.1, 1, 10 or 100 nM) for 6 or 15 h; then, their meiotic and developmental competence was evaluated using a number of cytological quality markers. With the exception of the highest dose (100 nM), the addition of 2-OHE₂ to the IVM m

SUBMITTER: Merico V 

PROVIDER: S-EPMC7782423 | biostudies-literature | 2021 Jan

REPOSITORIES: biostudies-literature