Suppression of pancreatic cancer liver metastasis by secretion-deficient ITIH5.

ABSTRACT:

Background

Previously, we identified ITIH5 as a suppressor of pancreatic ductal adenocarcinoma (PDAC) metastasis in experimental models. Expression of ITIH5 correlated with decreased cell motility, invasion and metastasis without significant inhibition of primary tumour growth. Here, we tested whether secretion of ITIH5 is required to suppress liver metastasis and sought to understand the role of ITIH5 in human PDAC.

Methods

We expressed mutant ITIH5 with deletion of the N-terminal secretion sequence (ITIH5Δs) in highly metastatic human PDAC cell lines. We used a human tissue microarray (TMA) to compare ITIH5 levels in uninvolved pancreas, primary and metastatic PDAC.

Results

Secretion-deficient ITIH5Δs was sufficient to suppress liver metastasis. Similar to secreted

SUBMITTER: Young ED 

PROVIDER: S-EPMC7782545 | biostudies-literature | 2021 Jan

REPOSITORIES: biostudies-literature