The order and logic of CD4 versus CD8 lineage choice and differentiation in mouse thymus.

ABSTRACT: CD4 and CD8 mark helper and cytotoxic T cell lineages, respectively, and serve as coreceptors for MHC-restricted TCR recognition. How coreceptor expression is matched with TCR specificity is central to understanding CD4/CD8 lineage choice, but visualising coreceptor gene activity in individual selection intermediates has been technically challenging. It therefore remains unclear whether the sequence of coreceptor gene expression in selection intermediates follows a stereotypic pattern, or is responsive to signaling. Here we use single cell RNA sequencing (scRNA-seq) to classify mouse thymocyte selection intermediates by coreceptor gene expression. In the unperturbed thymus, Cd4⁺Cd8a^- selection intermediates appear before Cd4^-Cd8a⁺ selection inter

SUBMITTER: Karimi MM 

PROVIDER: S-EPMC7782583 | biostudies-literature | 2021 Jan

REPOSITORIES: biostudies-literature