Error correction enables use of Oxford Nanopore technology for reference-free transcriptome analysis.

ABSTRACT: Oxford Nanopore (ONT) is a leading long-read technology which has been revolutionizing transcriptome analysis through its capacity to sequence the majority of transcripts from end-to-end. This has greatly increased our ability to study the diversity of transcription mechanisms such as transcription initiation, termination, and alternative splicing. However, ONT still suffers from high error rates which have thus far limited its scope to reference-based analyses. When a reference is not available or is not a viable option due to reference-bias, error correction is a crucial step towards the reconstruction of the sequenced transcripts and downstream sequence analysis of transcripts. In this paper, we present a novel computational method to error correct ONT cDNA sequencing data, called isONc

SUBMITTER: Sahlin K 

PROVIDER: S-EPMC7782715 | biostudies-literature | 2021 Jan

REPOSITORIES: biostudies-literature