Simultaneous Zn²⁺ tracking in multiple organelles using super-resolution morphology-correlated organelle identification in living cells.

ABSTRACT: Zn²⁺ plays important roles in metabolism and signaling regulation. Subcellular Zn²⁺ compartmentalization is essential for organelle functions and cell biology, but there is currently no method to determine Zn²⁺ signaling relationships among more than two different organelles with one probe. Here, we report simultaneous Zn²⁺ tracking in multiple organelles (Zn-STIMO), a method that uses structured illumination microscopy (SIM) and a single Zn²⁺ fluorescent probe, allowing super-resolution morphology-correlated organelle identification in living cells. To guarantee SIM imaging quality for organelle identification, we develop a new turn-on Zn²⁺ fluorescent probe, NapBu-BPEA, by regulating the lipophilicity of naphthalimide-deri

SUBMITTER: Fang H 

PROVIDER: S-EPMC7782730 | biostudies-literature | 2021 Jan

REPOSITORIES: biostudies-literature