In vivo nuclear RNA structurome reveals RNA-structure regulation of mRNA processing in plants.

ABSTRACT:

Background

mRNA processing is critical for gene expression. A challenge in regulating mRNA processing is how to recognize the actual mRNA processing sites, such as splice and polyadenylation sites, when the sequence content is insufficient for this purpose. Previous studies suggested that RNA structure affects mRNA processing. However, the regulatory role of RNA structure in mRNA processing remains unclear.

Results

Here, we perform in vivo selective 2'-hydroxyl acylation analyzed by primer extension (SHAPE) chemical profiling on Arabidopsis and generate the in vivo nuclear RNA structure landscape. We find that nuclear mRNAs fold differently from cytosolic mRNAs across translation start and stop sites. Notably, we discover a two-nucleotide single-stranded RNA structure featur

SUBMITTER: Liu Z 

PROVIDER: S-EPMC7784297 | biostudies-literature | 2021 Jan

REPOSITORIES: biostudies-literature