Disome-seq reveals widespread ribosome collisions that promote cotranslational protein folding.

ABSTRACT:

Background

The folding of proteins is challenging in the highly crowded and sticky environment of a cell. Regulation of translation elongation may play a crucial role in ensuring the correct folding of proteins. Much of our knowledge regarding translation elongation comes from the sequencing of mRNA fragments protected by single ribosomes by ribo-seq. However, larger protected mRNA fragments have been observed, suggesting the existence of an alternative and previously hidden layer of regulation.

Results

In this study, we performed disome-seq to sequence mRNA fragments protected by two stacked ribosomes, a product of translational pauses during which the 5'-elongating ribosome collides with the 3'-paused one. We detected widespread ribosome collisions that are related to slow

SUBMITTER: Zhao T 

PROVIDER: S-EPMC7784341 | biostudies-literature | 2021 Jan

REPOSITORIES: biostudies-literature