TSGIT: An N- and C-terminal tandem tag system for purification of native and intein-mediated ligation-ready proteins.

ABSTRACT: A large variety of fusion tags have been developed to improve protein expression, solubilization, and purification. Nevertheless, these tags have been combined in a rather limited number of composite tags and usually these composite tags have been dictated by traditional commercially-available expression vectors. Moreover, most commercially-available expression vectors include either N- or C-terminal fusion tags but not both. Here, we introduce TSGIT, a fusion-tag system composed of both N- and a C-terminal composite fusion tags. The system includes two affinity tags, two solubilization tags and two cleavable tags distributed at both termini of the protein of interest. Therefore, the N- and the C-terminal composite fusion tags in TSGIT are fully orthogonal in terms of both affinity selecti

SUBMITTER: Raducanu VS 

PROVIDER: S-EPMC7784762 | biostudies-literature | 2021 Feb

REPOSITORIES: biostudies-literature