Activity-based cell sorting reveals responses of uncultured archaea and bacteria to substrate amendment.

ABSTRACT: Metagenomic studies have revolutionized our understanding of the metabolic potential of uncultured microorganisms in various ecosystems. However, many of these genomic predictions have yet to be experimentally tested, and the functional expression of genomic potential often remains unaddressed. In order to obtain a more thorough understanding of cell physiology, novel techniques capable of testing microbial metabolism under close to in situ conditions must be developed. Here, we provide a benchmark study to demonstrate that bioorthogonal non-canonical amino acid tagging (BONCAT) in combination with fluorescence-activated cell sorting (FACS) and 16S rRNA gene sequencing can be used to identify anabolically active members of a microbial community incubated in the presence of various growth s

SUBMITTER: Reichart NJ 

PROVIDER: S-EPMC7784905 | biostudies-literature | 2020 Nov

REPOSITORIES: biostudies-literature