Mechanosensitive Ca²⁺ signaling and coordination is diminished in osteocytes of aged mice during ex vivo tibial loading.

ABSTRACT: Purpose: The osteocyte is considered the major mechanosensor in bone, capable of detecting forces at a cellular level to coordinate bone formation and resorption. The pathology of age-related bone loss, a hallmark of osteoporosis, is attributed in part to impaired osteocyte mechanosensing. However, real-time evidence of the effect of aging on osteocyte responses to mechanical load is lacking. Intracellular calcium (Ca²⁺) oscillations have been characterized as an early mechanosensitive response in osteocytes in systems of multiple scales and thus can serve as a real-time measure of osteocyte mechanosensitivity. Our objective was to utilize an ex vivo model to investigate potentially altered mechanosensing in the osteocyte network with aging.Methods: Tibiae w

SUBMITTER: Morrell AE 

PROVIDER: S-EPMC7785095 | biostudies-literature | 2020 May-Jul

REPOSITORIES: biostudies-literature