Differential Expression of Urinary Exosomal Small RNAs in Idiopathic Membranous Nephropathy.

ABSTRACT:

Background

Idiopathic membranous nephropathy (IMN) is a major cause of adult nephrotic syndromes, and reliable noninvasive biomarkers for diagnosis and monitoring are urgently needed. In this study, we performed small RNA (sRNA) sequencing to explore sRNA profiles of urinary exosomes derived from IMN patients and healthy controls (CON) to provide clues for identifying novel noninvasive sRNA biomarkers for IMN.

Methods

Urine samples were collected from five healthy controls and six patients with IMN. High-throughput sequencing was used to screen sRNA expression profiles of urinary exosomes from patients with IMN in two independent cohorts.

Results

Urinary exosomes were successfully isolated and used to obtain exosomal sRNAs. We screened 131 differentially expressed miR

SUBMITTER: Zhang J 

PROVIDER: S-EPMC7785370 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature