Genomic profiling of T-cell activation suggests increased sensitivity of memory T cells to CD28 costimulation.

ABSTRACT: T-cell activation is a critical driver of immune responses. The CD28 costimulation is an essential regulator of CD4 T-cell responses, however, its relative importance in naive and memory T cells is not fully understood. Using different model systems, we observe that human memory T cells are more sensitive to CD28 costimulation than naive T cells. To deconvolute how the T-cell receptor (TCR) and CD28 orchestrate activation of human T cells, we stimulate cells using varying intensities of TCR and CD28 and profiled gene expression. We show that genes involved in cell cycle progression and division are CD28-driven in memory cells, but under TCR control in naive cells. We further demonstrate that T-helper differentiation and cytokine expression are controlled by CD28. Using chromatin accessibil

SUBMITTER: Glinos DA 

PROVIDER: S-EPMC7785515 | biostudies-literature | 2020 Dec

REPOSITORIES: biostudies-literature