Tools for visualizing and analyzing Fourier space sampling in Cryo-EM.

ABSTRACT: A complete understanding of how an orientation distribution contributes to a cryo-EM reconstruction remains lacking. It is necessary to begin critically assessing the set of views to gain an understanding of its effect on experimental reconstructions. Toward that end, we recently suggested that the type of orientation distribution may alter resolution measures in a systematic manner. We introduced the sampling compensation factor (SCF), which incorporates how the collection geometry might change the spectral signal-to-noise ratio (SSNR), irrespective of the other experimental aspects. We show here that knowledge of the sampling restricted to spherical surfaces of sufficiently large radii in Fourier space is equivalent to knowledge of the set of projection views. Moreover, the SCF geometric

SUBMITTER: Baldwin PR 

PROVIDER: S-EPMC7785567 | biostudies-literature | 2021 Mar

REPOSITORIES: biostudies-literature