Novel Methylated DNA Markers in the Surveillance of Colorectal Cancer Recurrence.

ABSTRACT:

Purpose

We aimed to assess the concordance of colorectal cancer-associated methylated DNA markers (MDM) in primary and metastatic colorectal cancer for feasibility in detection of distantly recurrent/metastatic colorectal cancer in plasma.

Experimental design

A panel of previously discovered colorectal cancer-associated MDMs was selected. MDMs from primary and paired metastatic colorectal cancer tissue were assayed with quantitative methylation-specific PCR. Plasma MDMs were measured blindly by target enrichment long-probe quantitative-amplified signal assays. Random forest modeling was used to derive a prediction algorithm of MDMs in archival plasma samples from primary colorectal cancer cases. This algorithm was validated in prospectively collected plasma samples from recu

SUBMITTER: Xie H 

PROVIDER: S-EPMC7785570 | biostudies-literature | 2021 Jan

REPOSITORIES: biostudies-literature