Lipase from Rhizopus oryzae R1: in-depth characterization, immobilization, and evaluation in biodiesel production.

ABSTRACT:

Background

Rhizopus species is among the most well-known lipase producers, and its enzyme is suitable for use in many industrial applications. Our research focuses on the production of lipase utilizing waste besides evaluating its applications.

Results

An extracellular lipase was partially purified from the culture broth of Rhizopus oryzae R1 isolate to apparent homogeneity using ammonium sulfate precipitation followed by desalting via dialysis. The partially purified enzyme was non-specific lipase and the utmost activity was recorded at pH 6, 40 °C with high stability for 30 min. The constants K_m and V_max, calculated from the Lineweaver-Burk plot, are 0.3 mg/mL and 208.3 U/mL, respectively. Monovalent metal ions such as Na⁺ (1 and 5 mM) and

SUBMITTER: Helal SE 

PROVIDER: S-EPMC7785608 | biostudies-literature | 2021 Jan

REPOSITORIES: biostudies-literature