Changes in Cecal Microbiota and Short-chain Fatty Acid During Lifespan of the Rat.

ABSTRACT:

Background/aims

The gut microbiota regulates intestinal immune homeostasis through host-microbiota interactions. Multiple factors affect the gut microbiota, including age, sex, diet, and use of drugs. In addition, information on gut microbiota differs depending on the samples. The aim of this study is to investigate whether changes in cecal microbiota depend on aging.

Methods

Gut microbiota in cecal contents of 6-, 31-, and 74-week-old and 2-year-old male Fischer-344 rats (corresponding to 5-, 30-, 60-, and 80-year-old humans in terms of age) were analyzed using 16S ribosomal RNA metagenome sequencing and phylogenetic investigation of communities by reconstruction of unobserved states (PICRUSt) based on the Kyoto Encyclopedia of Genes and Genomes orthology. Moreover, short-c

SUBMITTER: Choi SI 

PROVIDER: S-EPMC7786083 | biostudies-literature | 2021 Jan

REPOSITORIES: biostudies-literature