Cytoplasmic maturation in human oocytes: an ultrastructural study †.

ABSTRACT: Female fertility relies on successful egg development. Besides chromosome segregation, complex structural and biochemical changes in the cytoplasmic compartment are necessary to confer the female gamete the capacity to undergo normal fertilization and sustain embryonic development. Despite the profound impact on egg quality, morphological bases of cytoplasmic maturation remain largely unknown. Here, we report our findings from the ultrastructural analysis of 69 unfertilized human oocytes from 34 young and healthy egg donors. By comparison of samples fixed at three consecutive developmental stages, we explored how ooplasmic architecture changes during meiotic maturation in vitro. The morphometric image analysis supported observation that the major reorganization of cytoplasm occurs before p

SUBMITTER: Trebichalska Z 

PROVIDER: S-EPMC7786262 | biostudies-literature | 2021 Jan

REPOSITORIES: biostudies-literature