Integration of diverse DNA substrates by a casposase can be targeted to R-loops in vitro by its fusion to Cas9.

ABSTRACT: CRISPR systems build adaptive immunity against mobile genetic elements by DNA capture and integration catalysed by Cas1-Cas2 protein complexes. Recent studies suggested that CRISPR repeats and adaptation module originated from a novel type of DNA transposons called casposons. Casposons encode a Cas1 homologue called casposase that alone integrates into target molecules single and double-stranded DNA containing terminal inverted repeats (TIRs) from casposons. A recent study showed Methanosarcina mazei casposase is able to integrate random DNA oligonucleotides, followed up in this work using Acidoprofundum boonei casposase, from which we also observe promiscuous substrate integration. Here we first show that the substrate flexibility of Acidoprofundum boonei casposase extends to random integ

SUBMITTER: Lau CH 

PROVIDER: S-EPMC7786333 | biostudies-literature | 2021 Jan

REPOSITORIES: biostudies-literature