Microglial deletion and inhibition alleviate behavior of post-traumatic stress disorder in mice.

ABSTRACT:

Background

Alteration of immune status in the central nervous system (CNS) has been implicated in the development of post-traumatic stress disorder (PTSD). However, the nature of overall changes in brain immunocyte landscape in PTSD condition remains unclear.

Methods

We constructed a mouse PTSD model by electric foot-shocks followed by contextual reminders and verified the PTSD-related symptoms by behavior test (including contextual freezing test, open-field test, and elevated plus maze test). We examined the immunocyte panorama in the brains of the naïve or PTSD mice by using single-cell mass cytometry. Microglia number and morphological changes in the hippocampus, prefrontal cortex, and amygdala were analyzed by histopathological methods. The gene expression changes of tho

SUBMITTER: Li S 

PROVIDER: S-EPMC7786489 | biostudies-literature | 2021 Jan

REPOSITORIES: biostudies-literature