Ancestral Sequence Reconstructions of MotB Are Proton-Motile and Require MotA for Motility.

ABSTRACT: The bacterial flagellar motor (BFM) is a nanomachine that rotates the flagellum to propel many known bacteria. The BFM is powered by ion transit across the cell membrane through the stator complex, a membrane protein. Different bacteria use various ions to run their BFM, but the majority of BFMs are powered by either proton (H⁺) or sodium (Na⁺) ions. The transmembrane (TM) domain of the B-subunit of the stator complex is crucial for ion selectivity, as it forms the ion channel in complex with TM3 and TM4 of the A-subunit. In this study, we reconstructed and engineered thirteen ancestral sequences of the stator B-subunit to evaluate the functional properties and ionic power source of the stator proteins at reconstruction nodes to evaluate the potential of ancestral seq

SUBMITTER: Islam MI 

PROVIDER: S-EPMC7787011 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature