A digital 3D reference atlas reveals cellular growth patterns shaping the Arabidopsis ovule.

ABSTRACT: A fundamental question in biology is how morphogenesis integrates the multitude of processes that act at different scales, ranging from the molecular control of gene expression to cellular coordination in a tissue. Using machine-learning-based digital image analysis, we generated a three-dimensional atlas of ovule development in Arabidopsis thaliana, enabling the quantitative spatio-temporal analysis of cellular and gene expression patterns with cell and tissue resolution. We discovered novel morphological manifestations of ovule polarity, a new mode of cell layer formation, and previously unrecognized subepidermal cell populations that initiate ovule curvature. The data suggest an irregular cellular build-up of WUSCHEL expression in the primordium and new functions for IN

SUBMITTER: Vijayan A 

PROVIDER: S-EPMC7787667 | biostudies-literature | 2021 Jan

REPOSITORIES: biostudies-literature