Generation and Starch Characterization of Non-Transgenic BEI and BEIIb Double Mutant Rice (Oryza sativa) with Ultra-High Level of Resistant Starch.

ABSTRACT:

Background

Cereals high in resistant starch (RS) are gaining popularity, as their intake is thought to help manage diabetes and prediabetes. Number of patients suffering from diabetes is also increasing in Asian countries where people consume rice as a staple food, hence generation of practically growable high RS rice line has been anticipated. It is known that suppression of starch branching enzyme (BE) IIb increases RS content in cereals. To further increase RS content and for more practical use, we generated a non-transgenic be1 be2b double mutant rice (Oryza sativa) line, which completely lacked both proteins, by crossing a be1 mutant with a be2b mutant.

Results

The be1 be2b mutant showed a decrease in intermediate amylopectin chains and an increase in long amylopectin c

SUBMITTER: Miura S 

PROVIDER: S-EPMC7788159 | biostudies-literature | 2021 Jan

REPOSITORIES: biostudies-literature