PlGF knockdown attenuates hypoxia-induced stimulation of cell proliferation and glycolysis of lung adenocarcinoma through inhibiting Wnt/β-catenin pathway.

ABSTRACT:

Background

Angiogenic placental growth factor (PlGF) plays a role in hypoxia-induced angiogenesis. Here, we aimed to investigate the biological roles of PlGF in cell proliferation and glycolysis of lung adenocarcinoma (LUAD) and the underlying molecular mechanisms.

Methods

PlGF was knocked down in H358 and H1975 cells by lentiviruses, which were then cultured under hypoxia (90% N₂, 5%CO₂ and 5%O₂) for 24 h. PlGF was overexpressed in PC9 cells treated with XAV939, inhibitor of Wnt/β-catenin signaling pathway. PlGF-silencing H1975 cells were implanted into mice, and tumor xenografts were harvested and analyzed.

Results

Hypoxia treatment led to up-regulation of PlGF, C-myc, lactate dehydrogenase A (LDHA), and β-catenin, promotion of cell

SUBMITTER: Zhang W 

PROVIDER: S-EPMC7788771 | biostudies-literature | 2021 Jan

REPOSITORIES: biostudies-literature