The effect of high-fat diet and exercise on KISS-1/GPR54 expression in testis of growing rats.

ABSTRACT:

Purpose

To find the expression of KISS-1 and G protein-coupled receptor 54 in rats testis from PND 21st to 56th.

Method

128 three-week-old weaned rats underwent high-fat diet and exercise (60-70% VO_2max, 1 h/day, 5 days/week) intervention and were randomly divided into group C, CE, HC, or HE. Sample time points were set on the PND 21st, 35th, 43rd, and 56th. The testicular testosterone and the mRNA content, and protein content of KISS-1 and GPR54 in testis tissue were detected by ELISA, RT-qPCR, and Western blotting.

Result

(1) The protein of KISS-1 and GPR54 increased gradually during the growing period. KISS-1 mRNA peaked at 35D and GPR54 peaked at 43D. (2) High-fat diet affected the expression of the KISS-1/GPR54 system in rat testis and reduced the expr

SUBMITTER: Feng J 

PROVIDER: S-EPMC7788936 | biostudies-literature | 2021 Jan

REPOSITORIES: biostudies-literature