Browse
Submit Data
Databases
API
Help

Dataset Information

6 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Global proteome profiling of human livers upon ischemia/reperfusion treatment.

ABSTRACT: Hepatic ischemia/reperfusion (I/R) injury represents a major risk factor for liver transplantation and is related to graft dysfunction and acute/chronic rejection. However, a significant part of these processes remain poorly characterized. To reveal differences in the proteome during liver I/R injury, we collected human liver biopsy samples during hepatectomy before and after the Pringle maneuver and conducted a TMT-based proteomic analysis through quantitative high-throughput mass spectrometry. We used a fold-change threshold of 1.3 and a t-test p-value?

SUBMITTER: Cai H

PROVIDER: S-EPMC7788958 | biostudies-literature | 2021 Jan

REPOSITORIES: biostudies-literature

ACCESS DATA

Json Xml

Publications

Global proteome profiling of human livers upon ischemia/reperfusion treatment.

Cai Haijian H Qi Shunli S Yan Qi Q Ling Jun J Du Jian J Chen Lijian L

Clinical proteomics 20210106 1

Hepatic ischemia/reperfusion (I/R) injury represents a major risk factor for liver transplantation and is related to graft dysfunction and acute/chronic rejection. However, a significant part of these processes remain poorly characterized. To reveal differences in the proteome during liver I/R injury, we collected human liver biopsy samples during hepatectomy before and after the Pringle maneuver and conducted a TMT-based proteomic analysis through quantitative high-throughput mass spectrometry. ...[more]

PMID: 33407080

Similar Datasets

Global proteome profiling of human livers upon ischemia/reperfusion treatment

Project description:Hepatic ischemia/reperfusion (I/R) injury represents a major risk factor for liver transplantation and is related to graft dysfunction and acute/chronic rejection. However, a significant part of these processes remain poorly characterized. To reveal differences in the proteome during liver I/R injury, we collected human liver biopsy samples during hepatectomy before and after the Pringle maneuver and conducted a TMT-based proteomic analysis through quantitative high-throughput mass spectrometry. We used a fold-change threshold of 1.3 and a t-test p-value<0.05 as the criteria to identify 5,257 total quantifiable proteins. The levels of 142 proteins were increased, while the levels of 103 proteins were decreased in response to hepatic I/R treatment. Bioinformatic analysis further revealed that these differentially expressed proteins are mainly involved in multiple biological functions and enzyme-regulated metabolic pathways. Most proteins whose expression was changed are related to the defense, immune and inflammatory responses as well as lipid and steroid metabolic processes. The upregulated proteins were found to be involved in immunity and inflammatory responses, and downregulated proteins were enriched in metabolic pathways. This study therefore may provide a research direction for the design of new therapeutic strategies for hepatic ischemia/reperfusion injury.

2020-02-26 | PXD017751 | iProX

Global MicroRNA Expression Profiling of Mouse Livers following Ischemia-Reperfusion Injury at Different Stages.

Project description: Not available

| S-EPMC4747576 | biostudies-literature

Global microRNA expression profiling of mouse livers following ischemia-reperfusion injury at different stages

Project description:This SuperSeries is composed of the SubSeries listed below.

2015-10-01 | GSE72315 | GEO

Profiling the Murine SUMO Proteome in Response to Cardiac Ischemia and Reperfusion Injury.

Project description: Not available

| S-EPMC7731038 | biostudies-literature

Global microRNA expression profiling of mouse livers following ischemia-reperfusion injury at different stages. [miRNA]

Project description:Hepatic ischemia reperfusion injury is a dynamic process consisting of two stages: ischemia and reperfusion, and triggers a cascade of physiological and biochemical events. Given the important role of microRNAs in regulating gene expression, we analyzed gene expression changes in mouse livers at sham control, ischemia stage, and reperfusion stage. We generated global expression profiles of microRNA and mRNA genes in mouse livers subjected to ischemia reperfusion injury at the three stages, respectively. Comparison analysis showed that reperfusion injury had a distinct expression profile whereas the ischemia sample and the sham control were clustered together. Consistently, there are 69 differentially expressed microRNAs between the reperfusion sample and the sham control whereas 28 differentially expressed microRNAs between the ischemia sample and the sham control. We further identified two modes of microRNA expression changes in ischemia reperfusion injury. Functional analysis of both the differentially expressed microRNAs in the two modes and their target mRNAs revealed that ischemia injury impaired mitochondria function, nutrient consumption, and metabolism process. In contrast, reperfusion injury led to severe tissue inflammation that is predominantly an innate-immune response in the ischemia reperfusion process. Our staged analysis of gene expression profiles provides new insights into regulatory mechanisms of microRNAs in mouse hepatic ischemia reperfusion injury.

2015-10-01 | GSE72314 | GEO

Global microRNA expression profiling of mouse livers following ischemia-reperfusion injury at different stages. [mRNA]

2015-10-01 | GSE72312 | GEO

Critical Roles of Calpastatin in Ischemia/Reperfusion Injury in Aged Livers.

Project description: Not available

| S-EPMC8394464 | biostudies-literature

Neuron-autonomous transcriptome changes upon ischemia/reperfusion injury.

Project description: Not available

| S-EPMC5517505 | biostudies-literature

Autophagy suppresses age-dependent ischemia and reperfusion injury in livers of mice.

Project description: Not available

| S-EPMC3221865 | biostudies-literature