A novel method for isolation and culture of primary swine gastric epithelial cells.

ABSTRACT:

Background

Culturing primary epithelial cells has a major advantage over tumor-derived or immortalized cell lines as long as their functional phenotype and genetic makeup are mainly maintained. The swine model has shown to be helpful and reliable when used as a surrogate model for human diseases. Several porcine cell lines have been established based on a variety of tissues, which have shown to extensively contribute to the current understanding of several pathologies, especially cancer. However, protocols for the isolation and culture of swine gastric epithelial cells that preserve cell phenotype are rather limited. We aimed to develop a new method for establishing a primary epithelial cell culture from the fundic gland region of the pig stomach.

Results

Mechanical and enzy

SUBMITTER: Bautista-Amorocho H 

PROVIDER: S-EPMC7789315 | biostudies-literature | 2021 Jan

REPOSITORIES: biostudies-literature