Cloning and characterization of KoOsmotin from mangrove plant Kandelia obovata under cold stress.

ABSTRACT:

Background

Low temperature is a major abiotic stress that seriously limits mangrove productivity and distribution. Kandelia obovata is the most cold-resistance specie in mangrove plants, but little is known about the molecular mechanism underlying its resistance to cold. Osmotin is a key protein associated with abiotic and biotic stress response in plants but no information about this gene in K. obovata was reported.

Results

In this study, a cDNA sequence encoding osmotin, KoOsmotin (GenBank accession no. KP267758), was cloned from mangrove plant K. obovata. The KoOsmotin protein was composed of 221 amino acids and showed a calculated molecular mass of 24.11 kDa with pI 4.92. The KoOsmotin contained sixteen cysteine residues and an N-terminal signal peptide, which were commo

SUBMITTER: Fei J 

PROVIDER: S-EPMC7789355 | biostudies-literature | 2021 Jan

REPOSITORIES: biostudies-literature