Identification of a potential neutralizing linear epitope of hemagglutinin-neuraminidase in Newcastle disease virus.

ABSTRACT:

Background

The hemagglutinin-neuraminidase (HN) protein of Newcastle disease virus (NDV) is a major antigen that can induce protective antibodies in poultry. However, its antigenic epitopes have not been fully elucidated. Therefore, defining the linear epitopes of HN, especially neutralizing epitopes, will be useful for revealing its antigenic characterization.

Methods

In this study, we analyzed B-cell immunodominant epitopes (IDEs) of the HN protein from the vaccine strain LaSota using pepscan technology with LaSota-specific chicken hyperimmune antisera. We constructed IDEs-RFP plasmids and prepared anti-IDEs peptide mouse sera to identify IDEs through immunological tests. At last, the different diluted anti-IDE antisera were used in BHK-21 cells to perform the neutralizati

SUBMITTER: Jin Z 

PROVIDER: S-EPMC7789432 | biostudies-literature | 2021 Jan

REPOSITORIES: biostudies-literature