Physical mapping and InDel marker development for the restorer gene Rf₂ in cytoplasmic male sterile CMS-D8 cotton.

ABSTRACT:

Background

Cytoplasmic male sterile (CMS) with cytoplasm from Gossypium Trilobum (D8) fails to produce functional pollen. It is useful for commercial hybrid cotton seed production. The restore line of CMS-D8 containing Rf₂ gene can restore the fertility of the corresponding sterile line. This study combined the whole genome resequencing bulked segregant analysis (BSA) with high-throughput SNP genotyping to accelerate the physical mapping of Rf₂ locus in CMS-D8 cotton.

Methods

The fertility of backcross population ((sterile line×restorer line)×maintainer line) comprising of 1623 individuals was investigated in the field. The fertile pool (100 plants with fertile phenotypes, F-pool) and the sterile pool (100 plants with sterile phenotypes, S-pool) were co

SUBMITTER: Feng J 

PROVIDER: S-EPMC7789476 | biostudies-literature | 2021 Jan

REPOSITORIES: biostudies-literature