The role of JrPPOs in the browning of walnut explants.

ABSTRACT:

Background

Tissue culture is an effective method for the rapid breeding of seedlings and improving production efficiency, but explant browning is a key limiting factor of walnut tissue culture. Specifically, the polymerization of PPO-derived quinones that cause explant browning of walnut is not well understood. This study investigated explants of 'Zanmei' walnut shoot apices cultured in agar (A) or vermiculite (V) media, and the survival percentage, changes in phenolic content, POD and PPO activity, and JrPPO expression in explants were studied to determine the role of PPO in the browning of walnut explants.

Results

The results showed that the V media greatly reduced the death rate of explants, and 89.9 and 38.7% of the explants cultured in V media and A media survived, resp

SUBMITTER: Zhao S 

PROVIDER: S-EPMC7789580 | biostudies-literature | 2021 Jan

REPOSITORIES: biostudies-literature