Mutation of RORγT reveals a role for Th17 cells in both injury and recovery from renal ischemia-reperfusion injury.

ABSTRACT: To investigate T helper type 17 (Th17) cells in the setting of acute kidney injury, the gene encoding the master regulator of Th17 cell differentiation, that is, RAR-related orphan receptor-γ (RORγT), was mutated in Lewis rats using CRISPR/Cas9 technology. In response to 40 min of bilateral renal ischemia-reperfusion (I/R), RAR-related orphan receptor C (Rorc)^-/- rats were resistant to injury relative to wild-type Rorc^+/+ rats. This protection was associated with inhibition of IL-17 expression and reduced infiltration of CD4⁺ cells, CD8⁺ cells, B cells, and macrophages. To evaluate the effect of Th17 cells on repair, ischemia was increased to 50 min in Rorc^-/- rats. This maneuver equalized the initial level of inju

SUBMITTER: Mehrotra P 

PROVIDER: S-EPMC7789982 | biostudies-literature | 2020 Nov

REPOSITORIES: biostudies-literature