Development of a Multiplex Bead Assay for the Detection of Canine IgG₄ Antibody Responses to Guinea Worm.

ABSTRACT: Increased levels of guinea worm (GW) disease transmission among dogs in villages along the Chari River in Chad threaten the gains made by the GW Eradication Program. Infected dogs with preemergent worm blisters are difficult to proactively identify. If these dogs are not contained, blisters can burst upon submersion in water, leading to the contamination of the water supply with L1 larvae. Guinea worm antigens previously identified using sera from human dracunculiasis patients were coupled to polystyrene beads for multiplex bead assay analysis of 41 non-endemic (presumed negative) dog sera and 39 sera from GW-positive dogs from Chad. Because commercially available anti-dog IgG secondary antibodies did not perform well in the multiplex assay, dog IgGs were partially purified, and a new anti

SUBMITTER: Priest JW 

PROVIDER: S-EPMC7790091 | biostudies-literature | 2021 Jan

REPOSITORIES: biostudies-literature