Spatiotemporal ATP Dynamics during AKI Predict Renal Prognosis.

ABSTRACT:

Background

Depletion of ATP in renal tubular cells plays the central role in the pathogenesis of kidney diseases. Nevertheless, inability to visualize spatiotemporal in vivo ATP distribution and dynamics has hindered further analysis.

Methods

A novel mouse line systemically expressing an ATP biosensor (an ATP synthase subunit and two fluorophores) revealed spatiotemporal ATP dynamics at single-cell resolution during warm and cold ischemic reperfusion (IR) with two-photon microscopy. This experimental system enabled quantification of fibrosis 2 weeks after IR and assessment of the relationship between the ATP recovery in acute phase and fibrosis in chronic phase.

Results

Upon ischemia induction, the ATP levels of proximal tubule (PT) cells decreased to the nadir

SUBMITTER: Yamamoto S 

PROVIDER: S-EPMC7790205 | biostudies-literature | 2020 Dec

REPOSITORIES: biostudies-literature