Transcriptional profiling of pediatric cholestatic livers identifies three distinct macrophage populations.

ABSTRACT:

Background & aims

Limited understanding of the role for specific macrophage subsets in the pathogenesis of cholestatic liver injury is a barrier to advancing medical therapy. Macrophages have previously been implicated in both the mal-adaptive and protective responses in obstructive cholestasis. Recently two macrophage subsets were identified in non-diseased human liver; however, no studies to date fully define the heterogeneous macrophage subsets during the pathogenesis of cholestasis. Here, we aim to further characterize the transcriptional profile of macrophages in pediatric cholestatic liver disease.

Methods

We isolated live hepatic immune cells from patients with biliary atresia (BA), Alagille syndrome (ALGS), and non-cholestatic pediatric liver by fluorescence activate

SUBMITTER: Taylor SA 

PROVIDER: S-EPMC7790256 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature