Live Imaging of Intracranial Lymphatics in the Zebrafish.

ABSTRACT:

Rationale

The recent discovery of meningeal lymphatics in mammals is reshaping our understanding of fluid homeostasis and cellular waste management in the brain, but visualization and experimental analysis of these vessels is challenging in mammals. Although the optical clarity and experimental advantages of zebrafish have made this an essential model organism for studying lymphatic development, the existence of meningeal lymphatics has not yet been reported in this species.

Objective

Examine the intracranial space of larval, juvenile, and adult zebrafish to determine whether and where intracranial lymphatic vessels are present.

Methods and results

Using high-resolution optical imaging of the meninges in living animals, we show that zebrafish possess a meningeal lymph

SUBMITTER: Castranova D 

PROVIDER: S-EPMC7790877 | biostudies-literature | 2021 Jan

REPOSITORIES: biostudies-literature