EGR1-mediated linc01503 promotes cell cycle progression and tumorigenesis in gastric cancer.

ABSTRACT:

Objectives

Long non-coding RNAs (lncRNAs) are key mediators in various malignancies. Linc01503 was previously elucidated to promote gastric cancer (GC) cell invasion. However, the upstream mechanism of linc01503 and its involvement in GC cell cycle, apoptosis and tumorigenesis still remain unclear.

Materials and methods

Bioinformatics analysis and quantitative reverse transcription polymerase chain reaction (qRT-PCR) assays were implicated to detect linc01503 level in GC. The role of linc01503 was detected by in vitro functional assays and in vivo xenograft tumour models. The association between linc01503 and its upstream effector was identified by chromatin immunoprecipitation (ChIP) assays. The mechanistic model of linc01503 was clarified using subcellular separation, fluo

SUBMITTER: Ma Z 

PROVIDER: S-EPMC7791171 | biostudies-literature | 2021 Jan

REPOSITORIES: biostudies-literature