BCL2 enhances survival of porcine pluripotent stem cells through promoting FGFR2.

ABSTRACT:

Objectives

The establishment of porcine pluripotent stem cells (pPSCs) is still a critical topic. However, all pPSCs were failed to contribute to efficient chimeric pig and were extremely sensitive to changes of culture conditions. This study aimed to investigate the role of BCL2 in pPSCs and further explain the mechanism.

Materials and methods

Porcine BCL2 gene was cloned and overexpressed in porcine induce pluripotent stem cells (piPSCs). Digital RNA-seq was performed to explain the mechanism of anti-apoptosis. Finally, the cells carrying BCL2 were injected into mouse early embryo to evaluate its chimeric ability.

Results

Here, we found that overexpression of porcine BCL2 gene significantly improved the survivability of piPSCs and the efficiency of embryonic chimeri

SUBMITTER: Zhu Z 

PROVIDER: S-EPMC7791183 | biostudies-literature | 2021 Jan

REPOSITORIES: biostudies-literature