Protective effect of hydrogen sulfide on endothelial cells through Sirt1-FoxO1-mediated autophagy.

ABSTRACT:

Background

As a new member of the vasculoprotective gasotransmitter family, hydrogen sulfide (H₂S) functions similar to nitric oxide (NO) and carbon monoxide (CO). Endothelial cell (EC) death and autophagy enable cells to cope with the progression of cardiovascular diseases. However, the impacts and underlying mechanisms of H₂S in the autophagic process in ECs are not completely understood. Here, we investigated the effects of H₂S on autophagy in human vascular ECs.

Methods

Human umbilical vein endothelial cells (HUVECs) were exposed to different concentrations (0, 50, 100, 200, 500 and 1,000 µmol/L) GYY4137 (H₂S donor) for indicated times (0, 0.5, 1, 2, 4 and 8 h), with or without pre-treatment with the autophagy inhibitor 3-methy

SUBMITTER: Zhu L 

PROVIDER: S-EPMC7791216 | biostudies-literature | 2020 Dec

REPOSITORIES: biostudies-literature