Ultrastructural alteration of pulmonary tissue under conditions of high oxygen concentration.

ABSTRACT:

Objective

To determine the structure of pulmonary tissue under conditions of high oxygen concentration.

Methods

Ten-week-old C57BL male mice and control mice were exposed to 100% oxygen and to room air for 72 hours, respectively. To follow the progression of lesions, the mice were sacrificed at 6, 12, 24, 48, and 72 hours after 100% oxygen administration. Lung specimens obtained from these mice underwent morphologic analysis and immunofluorescence studies. We used scanning and transmission electron microscopy to determine the ultrastructure of the pulmonary capillaries, including the endothelial glycocalyx. To visualize the endothelial glycocalyx, we performed lanthanum nitrate staining.

Results

The survival rate of the 100% oxygen administration group was 5% (2/40) a

SUBMITTER: Yano H 

PROVIDER: S-EPMC7791376 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature