PEPCK-M recoups tumor cell anabolic potential in a PKC-ζ-dependent manner.

ABSTRACT:

Background

Mitochondrial phosphoenolpyruvate carboxykinase (PEPCK-M; PCK2) is expressed in all cancer types examined and in neuroprogenitor cells. The gene is upregulated by amino acid limitation and ER-stress in an ATF4-dependent manner, and its activity modulates the PEP/Ca²⁺ signaling axis, providing clear arguments for a functional relationship with metabolic adaptations for cell survival. Despite its potential relevance to cancer metabolism, the mechanisms responsible for its pro-survival activity have not been completely elucidated.

Methods

[U-¹³C]glutamine and [U-¹³C]glucose labeling of glycolytic and TCA cycle intermediates and their anabolic end-products was evaluated quantitatively using LC/MS and GC/MS in conditions of abundant glu

SUBMITTER: Hyrossova P 

PROVIDER: S-EPMC7791766 | biostudies-literature | 2021 Jan

REPOSITORIES: biostudies-literature