Norwogonin attenuates hypoxia-induced oxidative stress and apoptosis in PC12 cells.

ABSTRACT:

Background

Norwogonin is a natural flavone with three phenolic hydroxyl groups in skeletal structure and has excellent antioxidant activity. However, the neuroprotective effect of norwogonin remains unclear. Here, we investigated the protective capacity of norwogonin against oxidative damage elicited by hypoxia in PC12 cells.

Methods

The cell viability and apoptosis were examined by MTT assay and Annexin V-FITC/PI staining, respectively. Reactive oxygen species (ROS) content was measured using DCFH-DA assay. Lactate dehydrogenase (LDH), malondialdehyde (MDA) and antioxidant enzyme levels were determined using commercial kits. The expression of related genes and proteins was measured by real-time quantitative PCR and Western blotting, respectively.

Results

We found tha

SUBMITTER: Jing L 

PROVIDER: S-EPMC7791982 | biostudies-literature | 2021 Jan

REPOSITORIES: biostudies-literature