Novel insights in dimethyl carbonate-based extraction of polyhydroxybutyrate (PHB).

ABSTRACT:

Background

Plastic plays a crucial role in everyday life of human living, nevertheless it represents an undeniable source of land and water pollution. Polyhydroxybutyrate (PHB) is a bio-based and biodegradable polyester, which can be naturally produced by microorganisms capable of converting and accumulating carbon as intracellular granules. Hence, PHB-producing strains stand out as an alternative source to fossil-derived counterparts. However, the extraction strategy affects the recovery efficiency and the quality of PHB. In this study, PHB was produced by a genetically modified Escherichia coli strain and successively extracted using dimethyl carbonate (DMC) and ethanol as alternative solvent and polishing agent to chloroform and hexane. Eventually, a Life Cycle Assessment (LCA)

SUBMITTER: Mongili B 

PROVIDER: S-EPMC7792028 | biostudies-literature | 2021 Jan

REPOSITORIES: biostudies-literature