Solid-state NMR of unlabeled plant cell walls: high-resolution structural analysis without isotopic enrichment.

ABSTRACT:

Background

Multidimensional solid-state nuclear magnetic resonance (ssNMR) spectroscopy has emerged as an indispensable technique for resolving polymer structure and intermolecular packing in primary and secondary plant cell walls. Isotope (¹³C) enrichment provides feasible sensitivity for measuring 2D/3D correlation spectra, but this time-consuming procedure and its associated expenses have restricted the application of ssNMR in lignocellulose analysis.

Results

Here, we present a method that relies on the sensitivity-enhancing technique Dynamic Nuclear Polarization (DNP) to eliminate the need for ¹³C-labeling. With a 26-fold sensitivity enhancement, a series of 2D ¹³C-¹³C correlation spectra were successfully collected using the u

SUBMITTER: Zhao W 

PROVIDER: S-EPMC7792314 | biostudies-literature | 2021 Jan

REPOSITORIES: biostudies-literature