Effect of overexpression of SNF1 on the transcriptional and metabolic landscape of baker's yeast under freezing stress.

ABSTRACT:

Background

Freezing stress is the key factor that affecting the cell activity and fermentation performance of baker's yeast in frozen dough production. Generally, cells protect themselves from injury and maintain metabolism by regulating gene expression and modulating metabolic patterns in stresses. The Snf1 protein kinase is an important regulator of yeast in response to stresses. In this study, we aim to study the role of the catalytic subunit of Snf1 protein kinase in the cell tolerance and dough leavening ability of baker's yeast during freezing. Furthermore, the effects of SNF1 overexpression on the global gene expression and metabolite profile of baker's yeast before and after freezing were analysed using RNA-sequencing and untargeted UPLC - QTOF-MS/MS, respectively.

Resul

SUBMITTER: Meng L 

PROVIDER: S-EPMC7792352 | biostudies-literature | 2021 Jan

REPOSITORIES: biostudies-literature