Serodiagnostic antigens of Clonorchis sinensis identified and evaluated by high-throughput proteogenomics.

ABSTRACT: Clonorchiasis caused by Clonorchis sinensis is endemic in East Asia; approximately 15 million people have been infected thus far. To diagnose the infection, serodiagnostic tests with excellent functionality should be performed. First, 607 expressed sequence tags encoding polypeptides with a secretory signal were expressed into recombinant proteins using an in vitro translation system. By protein array-based screening using C. sinensis-infected sera, 18 antigen candidate proteins were selected and assayed for cross-reactivity against Opisthorchis viverrini-infected sera. Of the six antigenic proteins selected, four were synthesized on large scale in vitro and evaluated for antigenicity against the flukes-infected human sera using ELISA. CsAg17 antigen showed the highest sensitivity (77.1%)

SUBMITTER: Cho PY 

PROVIDER: S-EPMC7793300 | biostudies-literature | 2020 Dec

REPOSITORIES: biostudies-literature