Bu-Yin-Qian-Zheng Formula Ameliorates MPP⁺-Induced Mitochondrial Dysfunction in Parkinson's Disease via Parkin.

ABSTRACT: As a typical traditional Chinese medicine, Bu-Yin-Qian-Zheng Formula (BYQZF) has been shown to have neuroprotective effects in patients with Parkinson's disease (PD), particularly by ameliorating mitochondrial dysfunction and regulating expression of the parkin protein. However, the underlying mechanisms by which BYQZF affects mitochondrial function through parkin are unclear. Accordingly, in this study, we evaluated the mechanisms by which BYQZF ameliorates mitochondrial dysfunction through parkin in PD. We constructed a parkin-knockdown cell model and performed fluorescence microscopy to observe transfected SH-SY5Y cells. Quantitative real-time reverse transcription polymerase chain reaction and western blotting were conducted to detect the mRNA and protein expression levels of parkin. A

SUBMITTER: Ma HJ 

PROVIDER: S-EPMC7793772 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature