Unraveling the 17β-Estradiol Degradation Pathway in Novosphingobium tardaugens NBRC 16725.

ABSTRACT: We have analyzed the catabolism of estrogens in Novosphingobium tardaugens NBRC 16725, which is able to use endocrine disruptors such as 17β-estradiol, estrone, and estriol as sole carbon and energy sources. A transcriptomic analysis enabled the identification of a cluster of catabolic genes (edc cluster) organized in two divergent operons that are involved in estrogen degradation. We have developed genetic tools for this estrogen-degrading bacterium, allowing us to delete by site-directed mutagenesis some of the genes of the edc cluster and complement them by using expression plasmids to better characterize their precise role in the estrogen catabolism. Based on these results, a catabolic pathway is proposed. The first enzyme of the pathway (17β-hydroxysteroid dehydro

SUBMITTER: Ibero J 

PROVIDER: S-EPMC7793797 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature